"Toe clipping is no longer necessary"
Transgenic Animals - Typical Genotyping Protocol
Facility not large enough to hold your expanding transgenic colony?
AIMS™ Neonate Tattoo Identification can eliminate the need for extra animal rooms!
Typical Protocol
- Tattoo pups on Day 3 - 9 using tail or paw pad tattooing
- Take tissue samples for DNA isolation (ear or tail)
- Perform genotype analysis on DNA from individually identified pups
- Cull animals that do not carry your gene
- Remaining transgenic pups grow faster and are healthier without the competition from non-transgenic pups
Tattooing neonates allows you to expand your transgenic colony with out having to:
- Wean large numbers of surplus mice
- House surplus mice
- Feed surplus mice
- Buy more caging
- Use more animal rooms
- Have higher perdium costs
- Use more personnel time
Benefits of AIMS™ Tattooing
- Tattoos are permanent
- Pigments are safe and non-toxic
- Tattooing will not injure pup
- Tattooing is easy and quick (Tattoo a litter within 3 minutes!)
- One system can tattoo neonate and adult mice
- On-site training is provided
- Used by transgenic facilities worldwide
- Cost effective - average 5 cents per mouse
- Practical alternative to toe clipping (non-invasive)
Neonate tattooing can be done without causing: pain, stress, or bleeding.
Tattoos are permanent and will not alter the physiology of your animals.
You can tattoo day 1 mice or rats!
